C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.(1) It has long been recognized that CRP is closely related to immunology, inflammation and host defense, as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.(2-4) Normally CRP is presenting only in a trace amount in circulation (<1 ug/ml)(5-6) but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 ug/ml, 1-3 ug/ml and >3 ug/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.(7) Therefore, blood CRP level has become a promising measure of CVD risk.(8-9)1. Thompson D., Pepys M.B. and Wood S.P. (1999) Structure, 7, 169-177.2. Festa A, D’Agostino R. Jr., Tracy R.P. and Haffner S.M. (2002) Diabetes, 51, 1131-1137.3. Verma S. and Yeh E.T. (2003) Am J Physiol, 285, R1253-R1258.4. Jialal I., Devaraj S. and Venugopal S.K. (2004) Hypertension, 44, 6-11.5. Kindmark C.O. (1972) Scand J Clin Lab Invest, 29, 407-411.6. Macy E.M., Hayes T.E. and Tracy R.P. (1997) Clin Chem, 43, 52-58.7. Ridker P.M. (2004) Am Heart Hosp J, 2(4 Suppl 1), 4-9.8. Benzaquen l.R., Yu H. and Rifai N. (2002) Crit Rev Clin Lab Sci, 39, 459-497.9. Pearson T.A. et al., (2003) Circulation, 107, 499-511.
新西兰Symansis公司是新西兰一家发展抗体作为新药开发中细胞讯息传递研究工具的公司。
Symansis公司新推出Multi-Kinase ELISA Arrays试剂盒,采用特异的抗磷酸化激酶抗体和ELISA反应原理,使磷酸化激酶的检测变得简单。一次操作,可同时检测多个磷酸化激酶,避免了传统的Western blot方法需要分开操作及其一系列复杂繁琐的操作和等待。其酶抑制剂产品如下:
AKT AKt-I-1 SY AKT-I-1
AMPK A-769662 SY-A-769662
AUR (CDK) JNJ7706621 SY- JNJ7706621
Aurora VX-680 SY- VX-680
Bcl-2 ABT-737 SY- ABT-737
BCR-ABL Imatinib mesylate SY- Imatinib mesylate
BCR-ABL PD173955 SY-PD173955
CDK AT-7519 SY- AT-7519
CDK SNS-032 SY- SNS-032
EGFR Gefitinib SY- Gefitinib
EGFR Erlotinib SY- Erlotinib