For the quantitative determination of human FGF-21 concentrations in serum, plasma and cell culture supernate samples.Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic ß-cells[3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents[5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon[6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance[7].[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635 [2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437 [3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203– 206 [4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687– 26695 [5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35. [6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81 [7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.
新西兰Symansis公司是新西兰一家发展抗体作为新药开发中细胞讯息传递研究工具的公司。
Symansis公司新推出Multi-Kinase ELISA Arrays试剂盒,采用特异的抗磷酸化激酶抗体和ELISA反应原理,使磷酸化激酶的检测变得简单。一次操作,可同时检测多个磷酸化激酶,避免了传统的Western blot方法需要分开操作及其一系列复杂繁琐的操作和等待。其酶抑制剂产品如下:
AKT AKt-I-1 SY AKT-I-1
AMPK A-769662 SY-A-769662
AUR (CDK) JNJ7706621 SY- JNJ7706621
Aurora VX-680 SY- VX-680
Bcl-2 ABT-737 SY- ABT-737
BCR-ABL Imatinib mesylate SY- Imatinib mesylate
BCR-ABL PD173955 SY-PD173955
CDK AT-7519 SY- AT-7519
CDK SNS-032 SY- SNS-032
EGFR Gefitinib SY- Gefitinib
EGFR Erlotinib SY- Erlotinib