IκB kinases (Ikks) are related kinases that play a major role in the regulation of NF-kB (nuclear factor kB) activation, a transcription factor activated in response to infectious agents and pro-inflammatory cytokines. NF-kB is sequestered in the cytoplasm in an inactive form by the IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB, thereby preventing translocation of NF-kB to the nucleus. Activation of NF-κB is mediated by phosphorylation of IκB on specific serine residues, which results in targeted proteasome-mediated degradation of IκB. This releases NF-kB dimers from the cytoplasmic NF-kB - IkB complex allowing NF-kB to translocate to the nucleus where it regulates the transcription of numerous target genes. The serine phosphorylation of IkB is regulated by the IKK complex. The IKK complex is comprised of at least three tightly associated subunits: IKKα, IKKβ and IKKγ. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit.
新西兰Symansis公司是新西兰一家发展抗体作为新药开发中细胞讯息传递研究工具的公司。
Symansis公司新推出Multi-Kinase ELISA Arrays试剂盒,采用特异的抗磷酸化激酶抗体和ELISA反应原理,使磷酸化激酶的检测变得简单。一次操作,可同时检测多个磷酸化激酶,避免了传统的Western blot方法需要分开操作及其一系列复杂繁琐的操作和等待。其酶抑制剂产品如下:
AKT AKt-I-1 SY AKT-I-1
AMPK A-769662 SY-A-769662
AUR (CDK) JNJ7706621 SY- JNJ7706621
Aurora VX-680 SY- VX-680
Bcl-2 ABT-737 SY- ABT-737
BCR-ABL Imatinib mesylate SY- Imatinib mesylate
BCR-ABL PD173955 SY-PD173955
CDK AT-7519 SY- AT-7519
CDK SNS-032 SY- SNS-032
EGFR Gefitinib SY- Gefitinib
EGFR Erlotinib SY- Erlotinib